Bismark

Bismark Processing Report

ZF230116-74_1.fastq.gz and ZF230116-74_2.fastq.gz

Data processed at 13:38:51 on 2023-03-24


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 461
Pairs without alignments under any condition 9352
Pairs that did not map uniquely 187
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 24900
Methylated C's in CpG context 503
Methylated C's in CHG context 344
Methylated C's in CHH context 1425
Methylated C's in Unknown context 1
Unmethylated C's in CpG context 279
Unmethylated C's in CHG context 4404
Unmethylated C's in CHH context 17945
Unmethylated C's in Unknown context 15
Percentage methylation (CpG context) 64.3%
Percentage methylation (CHG context) 7.2%
Percentage methylation (CHH context) 7.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 224 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 237 original bottom strand

Deduplication

Alignments analysed 461
Unique alignments 461
Duplicates removed 0
Duplicated alignments were found at 0 different positions

Cytosine Methylation after Extraction

Total C's analysed 16852
Methylated C's in CpG context 340
Methylated C's in CHG context 240
Methylated C's in CHH context 988
Unmethylated C's in CpG context 192
Unmethylated C's in CHG context 2974
Unmethylated C's in CHH context 12118
Percentage methylation (CpG context) 63.9%
Percentage methylation (CHG context) 7.5%
Percentage methylation (CHH context) 7.5%

M-Bias Plot